Practical Essay Example

Practical Essay Practical Name: Institution: Course: We will write a custom essay sample on Practical specifically for you for only $16.38 $13.9/page Order now We will write a custom essay sample on Practical specifically for you FOR ONLY $16.38 $13.9/page Hire Writer We will write a custom essay sample on Practical specifically for you FOR ONLY $16.38 $13.9/page Hire Writer Date: Practical Introduction The aim of embryo culture is to obtain a plant after isolating and growing an embryo in vitro. It involves the cultivation of embryos from seeds and ovules in aseptic conditions. The culture medium depends on the type of plants, and the age of the embryo. A person can use mature or immature embryos. People practice embryo culture for different reasons. Some do it because they want to know more about the plant. Growing the embryos outside the ovules gives a person the chance to learn the nutrient composition of the plant at every stage of development, identify the requirements of the plant and identify the different stages of growth. Embryo culture provides a way of getting hybrid seedlings and overcoming seed dormancy. This enables the production of crops with superior qualities. The practice of embryo culture is important because it used to recover distant hybrids and haploid plants. This practice is important because it shortens the breeding cycle of the plants (Bhojwani Razdan, 1 996). Materials and Methods We used snow peas pods and five plates of pea culture medium. We adjusted the culture medium to a pH of 5.7. Embryos grow well in a medium with a pH of 5 to 7 (Razdan, 2003). It contained 110g/L of vitamins, 11% sucrose, 50ml/L of coconut water containing agar, and casein hydrolysale. One needs to select the right culture medium that will support orderly and progressive development of the embryo. One should ensure that the medium is able to nourish the embryo. Sucrose is a source of energy and it supplements the vitamins. Using high quantity of vitamins can inhibit the growth. Therefore, one should use vitamins minimally. Other materials included scalpels, plates, grid paper, and aluminum foil. We rinsed the pods with water, and then sterilized them in 70% ethanol for two minutes. We then washed the pods again with distilled water and treated them with 2% hypochlorite solution. After the treatment, we washed the seeds again using distilled water. We opened the cotyledons, and removed the embryo hearts from the snow peas pods while exercising caution not to damage the embryos. We used a scalpel tip to cut the embryos horizontally into two parts. We measured the embryos using a grid paper. It is important and mandatory to measure the embryos before putting them in culture, so that one can determine the level of growth after the appointed time. We then photographed the embryos using the digital camera. We then put the two embryos in different culture media. We sealed the plates with the aluminum foil and incubated them at 22o for three weeks. After this time, we re-measured the embryos to check their growth. Results Embryo culture results- Initial Size (mm2)Final size (mm2)Difference (mm2) 2 25 23 2 25 23 3 35 32 1 10 9 4 20 16 9 30 21 9 25 16 10 30 20 8 36 28 12 150 138 4 60 56 Discussion There was only one healthy cotyledon. The other cotyledons were contaminated in different ways or damaged by heat. The contamination of the embryos may have happened during the cutting process. Although we sterilized and treated the embryos, we did not take the same effort in ensuring that the materials we were using were not contaminated. This affects the results of the culture. We did not get the results we had anticipated. The embryos did not grow as well as we had expected them to do. Some of the embryos were damaged by heat. We had sealed them with aluminum foil and put them under 22o. It seems that we exaggerated the level of heat required, or we used the wrong materials to seal the embryos. Contamination affects the growth of the embryos because it introduces bacteria. The bacteria breed on the embryos, and destroy the cells of the embryos. This hinders growth. We were not careful when extracting the embryos from the pods, and we saw this after seeing some of the damaged cotyl edons. This destroyed the embryonic tissues and prohibited the embryos development. We had used embryos of different sizes, ranging from 1 mm2 to 12 mm2. At the end of the experiment, the sizes of the final cotyledons did not correspond to the sizes of the embryos. However, we experienced the highest growth rate of 150 mm2 from the largest embryo, and the lowest growth rate of 10 mm2 from the smallest embryo. It seems that the contamination and the heat affected the growth of the embryos. Growth of the embryos occurs when there is cell division in the embryos. The development of the embryos occurs when cells specialize. This explains why there was growth in the cells, although they were contaminated, and why the largest embryos showed the highest growth rates. The size of the embryos determines the growth rate of the embryos. Large embryos have many cells, while the smaller embryos have fewer cells. Therefore, the larger embryos have a higher chance of developing (Kohl, 2007). References: Bhojwani, S. S., Razdan, K. M. (1996). Plant tissue culture: Theory and practice. Boston, MA: Elsevier Kohl, B. (2007). Embryo culture: Making babies in the twenty-first century. New York, NY: Farrar, Straus and Giroux Razdan, K. M. (2003). Introduction to plant tissue culture. Enfield, NH: Science Publishers, Inc.

Freedom Of The City Monologue Essay Example

Freedom Of The City Monologue Essay Example Freedom Of The City Monologue Paper Freedom Of The City Monologue Paper Dont ask me nothing, young feels. Ive no head. All do is march. And if you want to know why you should be marching you ask the buck inside. Me? Sure everybody was marching the day Well, for the same reason as everybody else. My reasons no different to anybody elses. Wan man wan vote -thats what I want. You know -wan man wan vote. Sure I know that. Sure I know we got it. Gerrymandering- thats another thing- no more gerrymandering- thats what I want- no more gerrymandering. And civil rights for everybody- thats what I ant- you know- civil rights- civil rights- thefts why march. Im a liar then? Youre calling me a liar, is that it? Hell be telling me my name isnt Lily Doherty next. Did you ever hear off Mongol child, Skinner? Told you a lie about our Declare. Thats what Declare is. Hess not just shy , our Declare. Hes a Mongol. And its for him I go on all the civil rights marches. Isnt that stupid? You and him and everybody else else marching and protesting about sensible things like politics and stuff and me in the middle of you all, marching for Declare. : Isnt that the stupidest thing you ever heard? Sure I could march and protest from here to Dublin and sure what good would it do Declare? Stupid and all as am know that much. But still march- every Sunday. Isnt that the stupidest thing you ever heard? Thats what the chairman said when l- you know- when tried to tell him what was thinking. He never talks about him; you cant even look at him. And that day thefts what he said, Youre a bone stupid bitchy. No wonder the kids bone stupid too. The chairman- thats what he said.

Labor Discrimination Essay Example | Topics and Well Written Essays - 2500 words

Labor Discrimination - Essay Example The intention of this study is labour discrimination refers to the discriminating behaviour practices within the process of hiring, promoting, assignment and allocation of job responsibilities and compensation to the employees. There are several types of harassment also included in labour discrimination like sexual harassment, cultural harassment and wages discrimination etc. There have been several jurisdiction implemented to prohibit different types of labour discrimination and the victims have been given the right to file lawsuit against the employers in case of encountering discrimination at work place. Labour discrimination could occur intentionally or intentionally due to prejudice or ignorance. The discrimination of labour has been evolved as an important and serious area of concern for the business related people, workers and general people because all over the world it is common to see filing of lawsuits against major companies of the world on the charges of labour discrimin ation.If the lawsuits are filed against well-known companies then the cases often turn sensational and gain the attention of the community to look for the outcome of the case. Several cases of labour discrimination have been ended with multimillion dollar settlement. The class actions taken against the companies are usually supported and favoured by the people because they think that decisions in favour of labour put pressure upon the employers to change their practice and make it justified and fair with the workforce. (Susan et al, p28). The paper discusses two lawsuits filed against popular American companies Abercrombie & Fitch and Wall Mark on the allegation of labour discrimination. These two cases were widely followed by people because they involved popular retail brands of the country. These cases ended up with different conclusion and the accused companies are given different decisions. Abercrombie & Fitch was ordered to pay $50 million compensate the plaintiffs however, the case of Wal-Mart was blocked by the Supreme Court due to lack of sufficient evidences about the existence of discriminating labour practice by the management of Wal-Mart. The decision of Abercrombie & Fitch encouraged the class workers to raise voice for their due rights however, the case of Wal-Mart clarifies that the lawsuit must be filed with complete preparation and practice so that the court become able to make a justified decision. These two important lawsuits of the U.S. business world are discussed below and their comparative analysis has also been conducted to illustrate the practical implications of these decisions to the business world and labour management practice. Abercrombie & Fitch Lawsuit Abercrombie & Fitch is a popular American retailer company that main deals with the casual wean for the consumers age group 18-22. It also operates three other offshoot brands of children wear and other fashion wear. The company has established around 300 locations across US and t he process of international expansion is also on its way. The company employs over 22,000 workers in over 700 stores. Most of the employees are college age adults of different sex, race and ethnic groups (Jane, p2). The case was filed by the employees of the company who charged it for discrimination of the work force at workplace. The case against Abercrombie & Fitch was settled on the 14th of April 2005 when Judge Susan Illston of U.S. District Court for the Northern district of California finally approved the settlement of case Gonzalez v. Abercrombie & Fitch. The court required Abercrombie & Fitch to pay $40 million to several plaintiffs that were mostly females and others belonged to minority community (Shiffer et al, p7). After the settlement of the case the company established settlement fund to distribute the amount among the workers. The plaintiffs were sent the award checks in